Journal: Microbial Cell Factories

Article Title: Design of an efficient medium for heterologous protein production in Yarrowia lipolytica: case of human interferon alpha 2b

doi: 10.1186/1475-2859-10-38

Figure Lengend Snippet: Effect of PTM1 concentration . (I-a) effect of PTM1 concentration on JMY1852p strain growth, hIFN α2b production using 2 ml/l of PTM1 solution (I-b) and 5 ml/l of PTM1 solution (I-c). Cultures were carried in 5-l bioreactor with oleic acid as carbon and inducer source, data represent the mean of duplicate. Open triangle: 5 ml PTM1 addition; closed triangle: 2 ml PTM1 addition. (II-a) a 28 kDa protease detected in cultures supernatants (SN). Lane 1; molecular weight, lane 2; SN (5 ml/l PTM1), lane 3; SN (2 ml/l PTM1). Protease inhibition by pepstatin addition at 10 μM (II-b) and 5 ml/l of PTM1 addition (II-c).

Article Snippet: Cultures were carried out in a 5-l bioreactor (Infors, Switzerland) with working volume of 2 l. After sterilization at 121°C for 30 min, the medium was inoculated with 200 ml of pre-culture at an initial OD 600 nm culture of 0.3.

Techniques: Concentration Assay, Molecular Weight, Inhibition

Journal: Scientific Reports

Article Title: Statistical optimization of P(3HB- co -3HHx) copolymers production by Cupriavidus necator PHB − 4/pBBR_CnPro- phaC Rp and its properties characterization

doi: 10.1038/s41598-023-36180-7

Figure Lengend Snippet: Batch fermentation profile of C. necator PHB − 4/pBBR_CnPro- phaC Rp p in 10L stirred-tank bioreactor.

Article Snippet: Fermentation was carried out in a 10 L stirred-tank bioreactor (Model MDFT-N-10L, Marubishi, Japan) to improve the production of P(3HB- co -3HHx) by C. necator PHB − 4/pBBR_CnPro- phaC Rp .

Techniques:

Journal: Scientific Reports

Article Title: Statistical optimization of P(3HB- co -3HHx) copolymers production by Cupriavidus necator PHB − 4/pBBR_CnPro- phaC Rp and its properties characterization

doi: 10.1038/s41598-023-36180-7

Figure Lengend Snippet: Proton Nuclear Magnetic Resonance Spectroscopy ( 1 H NMR) spectrum of P(3HB- co -3HHx) produced by C. necator PHB − 4/pBBR_CnPro- phaC Rp in 10L stirred-tank bioreactor.

Article Snippet: Fermentation was carried out in a 10 L stirred-tank bioreactor (Model MDFT-N-10L, Marubishi, Japan) to improve the production of P(3HB- co -3HHx) by C. necator PHB − 4/pBBR_CnPro- phaC Rp .

Techniques: Nuclear Magnetic Resonance, Spectroscopy, Produced

Journal: Scientific Reports

Article Title: Statistical optimization of P(3HB- co -3HHx) copolymers production by Cupriavidus necator PHB − 4/pBBR_CnPro- phaC Rp and its properties characterization

doi: 10.1038/s41598-023-36180-7

Figure Lengend Snippet: Attenuated Total Reflection Fourier transform infrared spectroscopy (ATR-FTIR) spectrum of P(3HB- co -3HHx) produced by C. necator PHB − 4/pBBR_CnPro- phaC Rp in 10L stirred-tank bioreactor.

Article Snippet: Fermentation was carried out in a 10 L stirred-tank bioreactor (Model MDFT-N-10L, Marubishi, Japan) to improve the production of P(3HB- co -3HHx) by C. necator PHB − 4/pBBR_CnPro- phaC Rp .

Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Produced

Journal: Scientific Reports

Article Title: Statistical optimization of P(3HB- co -3HHx) copolymers production by Cupriavidus necator PHB − 4/pBBR_CnPro- phaC Rp and its properties characterization

doi: 10.1038/s41598-023-36180-7

Figure Lengend Snippet: Differential Scanning Calorimetry (DSC) analysis of P(3HB- co -3HHx) produced by C. necator PHB − 4/pBBR_CnPro- phaC Rp in 10L stirred-tank bioreactor.

Article Snippet: Fermentation was carried out in a 10 L stirred-tank bioreactor (Model MDFT-N-10L, Marubishi, Japan) to improve the production of P(3HB- co -3HHx) by C. necator PHB − 4/pBBR_CnPro- phaC Rp .

Techniques: Differential Scanning Calorimetry, Produced

Journal: Scientific Reports

Article Title: Statistical optimization of P(3HB- co -3HHx) copolymers production by Cupriavidus necator PHB − 4/pBBR_CnPro- phaC Rp and its properties characterization

doi: 10.1038/s41598-023-36180-7

Figure Lengend Snippet: Thermogravimetric analysis (TGA) of P(3HB- co -3HHx) produced by C. necator PHB − 4/pBBR_CnPro- phaC Rp in 10L stirred-tank bioreactor.

Article Snippet: Fermentation was carried out in a 10 L stirred-tank bioreactor (Model MDFT-N-10L, Marubishi, Japan) to improve the production of P(3HB- co -3HHx) by C. necator PHB − 4/pBBR_CnPro- phaC Rp .

Techniques: Produced

Journal: Bioengineered

Article Title: Production of polyhydroxyalkanoates (PHAs) by Bacillus megaterium using food waste acidogenic fermentation-derived volatile fatty acids

doi: 10.1080/21655979.2021.1935524

Figure Lengend Snippet: Scheme showing the integration of VFAs production through acidogenic fermentation of food waste using membrane bioreactors with PHAs production by Bacillus megaterium ATCC 14,945 through shake flask experiments together with factors studied and analyses carried out in this work

Article Snippet: The volatile fatty acids stream was obtained from acidogenic fermentation of food waste using a continuous 2.5 L stirred-tank bioreactor (BBI-biotech GmbH, Berlin, Germany) equipped with a membrane panel (68.6 cm 2 effective area and average pore size of 0.3 µm) (VITO NV, Mol, Belgium).

Techniques:

Journal: PLoS ONE

Article Title: Improved Production Process for Native Outer Membrane Vesicle Vaccine against Neisseria meningitidis

doi: 10.1371/journal.pone.0065157

Figure Lengend Snippet: Process performance is evaluated with the NonaMen concept, a nonavalent PorA vaccine comprised of three trivalent RL production strains (Δ rmpM –Δ lpxL1 mutant strains; Supplementary ). Production of trivalent bulk NOMV is depicted in phases A to H. The transition from one phase to the next requires a specific unit operation (1 to 10). The seedlot (A) is first expanded in shake flask and bioreactor pre-cultures, then used to inoculate the production bioreactor. Harvested cells (B) are concentrated with microfiltration (C), and vesicle release is stimulated with a detergent-free buffer containing the chelating agent EDTA (D). Cells are discarded, and the crude NOMV are concentrated with ultrafiltration (E). Any residual DNA is digested with nuclease (F), and the extract is purified with gel filtration chromatography. The non-sterile bulk (G) is then sterilized by filtration to obtain the bulk NOMV (H). The trivalent product can be stored for at least one year before mixing as nonavalent vaccine and diluting to dose concentration (I).

Article Snippet: At OD 590 values between 1.5 and 4.5, a fixed amount of bacteria (corresponding to 1 L at OD 590 = 3) was transferred to the 60 L production bioreactor with 40 L working volume (Applikon, Schiedam, The Netherlands).

Techniques: Mutagenesis, Purification, Filtration, Chromatography, Sterility, Concentration Assay

Journal: PLoS ONE

Article Title: Improved Production Process for Native Outer Membrane Vesicle Vaccine against Neisseria meningitidis

doi: 10.1371/journal.pone.0065157

Figure Lengend Snippet: Specifications for improved NOMV production.

Article Snippet: At OD 590 values between 1.5 and 4.5, a fixed amount of bacteria (corresponding to 1 L at OD 590 = 3) was transferred to the 60 L production bioreactor with 40 L working volume (Applikon, Schiedam, The Netherlands).

Techniques: Centrifugation, Functional Assay, Extraction, Filtration, Concentration Assay, Chromatography, Sterility